Readthrough can be utilized in genetic circuit design to control protein expression
Readthrough mechanisms, such as conditional stop codon readthrough or suppressor tRNA usage, can be effectively employed in synthetic biology to achieve precise translational control over protein expression in genetic circuits.
The claim specifically asserts that readthrough can be used in genetic circuit design to control protein expression. Papers [3] and [9] directly discuss and demonstrate utilizing translational readthrough (via suppressor tRNAs and genetic code expansion) to precisely regulate and control gene expression in synthetic genetic circuits. Other papers discuss readthrough in the context of disease therapies or basic cellular quality control, but the supporting papers specifically validate the synthetic biology application mentioned in the claim.
Xiaotong Wang, Jianping Xu, Yipeng Wang, Qingsheng Qi, Zhiguo Wang, Qian Wang. Engineered suppressor tRNAs enable precise translational control of genetic circuits in E. coli.. 2026. https://doi.org/10.1093/nar/gkag623
Demonstrates that engineered suppressor tRNAs enable programmable nonsense mutation readthrough to provide precise translational control in synthetic genetic circuits.
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Yusuke Kato. Translational Control using an Expanded Genetic Code.. 2019. https://doi.org/10.3390/ijms20040887
Reviews how conditional stop codon readthrough via genetic code expansion and unnatural amino acids serves as an effective controller of target gene expression in synthetic gene circuits.
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