Achieving sterility in nucleic acid work requires specific enzymatic and chemical decontamination
Achieving and maintaining sterility or freedom from contamination in nucleic acid work frequently requires specialized enzymatic and chemical decontamination protocols, such as uracil-DNA-glycosylase or restriction enzyme treatments.
The retrieved literature consistently shows that preventing and eliminating contamination in nucleic acid amplification (like PCR and LAMP) requires specific enzymatic (e.g., UNG, restriction enzymes, CRISPR/Cas systems) and chemical strategies, directly supporting the claim.
Wei-Jou Lin, Tian Tian, Yongzhong Jiang, Erhu Xiong, Debin Zhu, Xiaoming Zhou. A CRISPR/Cas9 eraser strategy for contamination‐free PCR end‐point detection. 2021. https://doi.org/10.1002/bit.27718
Demonstrates the use of a CRISPR/Cas9 strategy to eliminate contamination in PCR systems.
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T. Quyen, A. C. Vinayaka, Mohsen Golabi, Huynh Van Ngoc, D. D. Bang, A. Wolff. Elimination of Carryover Contamination in Real-Time Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid Detection of the SARS-CoV-2 Virus in Point-of-Care Testing. 2022. https://doi.org/10.3389/fcimb.2022.856553
Shows the use of Cod-uracil-DNA-glycosylase to eliminate carryover contamination in nucleic acid amplification assays.
John Ashkenas, James W Dennis, Chi Yip Ho. Simple enzymatic means to neutralize DNA contamination in nucleic acid amplification.. 2005. https://doi.org/10.2144/05391ST02
Describes enzymatic methods such as restriction enzymes to neutralize DNA contamination in nucleic acid amplification.
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