Pan-assay interference compounds (PAINS) and frequent hitters are widely recognized in high-throughput screening for generating false positives and disrupting biological assays through nonspecific mechanisms.
The claim states that pan-assay interference compounds disrupt biological assays. The retrieved literature includes studies like [11], which specifically characterizes PAINS as nuisance compounds that interfere with biochemical or cell-based assays and generate misleading signals, and [0], which examines frequent hitters in high-throughput screening. Thus, the evidence directly supports the claim.