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the claim
Golden Gate assembly enables the joining of small DNA parts.
the verdict
SUPPORTED
the evidence backs this
Recorded sources
4 sources for · 0 against
Is it true that Golden Gate assembly enables the joining of small DNA parts?
Yes. Multiple studies demonstrate that Golden Gate assembly effectively joins small DNA parts, fragments, and modules into larger constructs in a single reaction.
Verdict: SUPPORTED · 4 sources for, 0 against · Last checked
The analysis
The retrieved papers consistently describe and utilize Golden Gate cloning/assembly as a standard technique for joining multiple DNA parts and fragments. Papers 0, 5, 7, and 11 explicitly confirm this capability across various applications in synthetic biology.
Evidence for · 4
Golden Gate cloning is described as a method used for the assembly of multiple DNA fragments in a defined linear order using a one-pot procedure.
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Marillonnet, S. & Grützner, R. (2020). Synthetic DNA Assembly Using Golden Gate Cloning and the Hierarchical Modular Cloning Pipeline. doi.org. https://doi.org/10.1002/cpmb.115
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More for · 3
CyanoConstruct utilizes a single Golden Gate reaction to assemble multi-gene expression plasmids from individual parts.
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OM, V., R, N., A, P., P, P., M, E., A, A., L, K., D, N., O, M., L, T., A, B., N, K., EM, A., KP, A., & P, K. (2026). CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.. doi.org. https://doi.org/10.1186/s12896-025-01098-7
Slowpoke automates Golden Gate-based cloning workflows for standardizing DNA assembly in synthetic biology.
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K, M., F, M., H, G., A, F. D. S., J, C., G, B., & T, E. (2026). Slowpoke: An Automated Golden Gate Cloning Workflow for Opentrons OT-2 and Flex.. doi.org. https://doi.org/10.1021/acssynbio.5c00629
Golden Gate vector assembly is integrated into protocols for generating targeted constructs in plant functional genomics.
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İ, E., R, D., M, S., İ, M., M, G., & M, T. (2026). Genome editing and regeneration pipeline for engineering disease resistance in tomato using CRISPR/Cas9.. doi.org. https://doi.org/10.3389/fpls.2026.1754287
Receipt History
first checked05 Aug 2026
judged → SUPPORTED · 8805 Aug 2026
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