Fluorouracil inhibits thymidylate synthase to suppress DNA synthesis

the verdict

SUPPORTED

the evidence backs this

Recorded sources

4 sources for · 0 against

Counts group repeated records of the same source within each side. They do not measure evidence strength or source independence.

Is it true that Fluorouracil inhibits thymidylate synthase to suppress DNA synthesis?

Yes. Multiple studies and reviews confirm that fluorouracil (5-FU) exerts its cytotoxic effects by inhibiting thymidylate synthase, which subsequently suppresses DNA synthesis and cancer cell proliferation.

Verdict: SUPPORTED · 4 sources for, 0 against · Last checked 5 Aug 2026

The analysis

The retrieved literature consistently supports the established pharmacological mechanism of 5-fluorouracil, noting that its active metabolite inhibits thymidylate synthase to disrupt thymidine production and suppress DNA synthesis.

Evidence for · 4

Ribosome Quality Control mitigates the cytotoxicity of ribosome collisions induced by 5-Fluorouracil

2024

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Chatterjee, S., Naeli, P., Onar, O., Simms, N., Garzia, A., Hackett, A. P., Coyle, K., Snell, P. H., McGirr, T., Sawant, T. N., Dang, K., Stoichkova, Z. V., Azam, Y., Saunders, M., Braun, M., Alain, T., Tuschl, T., McDade, S., Longley, D. B., Gkogkas, C. G., Adrain, C., Knight, J. R. P., & Jafarnejad, S. M. (2024). Ribosome Quality Control mitigates the cytotoxicity of ribosome collisions induced by 5-Fluorouracil. doi.org. https://doi.org/10.1093/nar/gkae849

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More for · 3

Structural aspects of the inhibition and catalytic mechanism of thymidylate synthase.

1995

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Hardy, L. W. (1995). Structural aspects of the inhibition and catalytic mechanism of thymidylate synthase.. doi.org. https://doi.org/10.18388/abp.1995_4891

Bridging mechanism and design: modern medicinal chemistry approaches to thymidylate synthase inhibitors.

2026

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AA, A., ME, E., TA, Y., AO, A. K., & SS, H. (2026). Bridging mechanism and design: modern medicinal chemistry approaches to thymidylate synthase inhibitors.. doi.org. https://doi.org/10.1039/d5ra08381h

Everolimus destabilizes thymidylate synthase via suppressing its O-GlcNAcylation and sensitizes HER2-negative breast cancer to fluorouracil.

2026

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XT, J., H, G., S, W., L, W., Z, C., LL, W., J, W., M, Z., N, L., W, Y., Y, L., L, J., Y, L., S, L., Q, S., J, L., K, Q., Q, W., X, Y., Z, Y., Y, C., Y, W., & Q, L. (2026). Everolimus destabilizes thymidylate synthase via suppressing its O-GlcNAcylation and sensitizes HER2-negative breast cancer to fluorouracil.. doi.org. https://doi.org/10.1038/s41419-026-08715-z

What does the evidence say?

Receipt History

first checked05 Aug 2026

judged → SUPPORTED · 8205 Aug 2026

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