Transcription starts from a specific end of the mRNA molecule
the verdict
SUPPORTED
the evidence backs this
refutedsupported
the weight of evidence
4 sources for · 0 against
Peer-reviewed literature demonstrates that transcription initiates at specific mapped start sites located at defined positions relative to reading frames or ends of molecules.
The nucleotide sequence of the manX(ptsL) gene, encoding enzyme IIIMan function in the phosphoenolpyruvate transport system, and that of the 5′ end of the manY(pel) gene of Escherichia coli, was determined. Sequence analysis of manX revealed the presence of a large open reading frame with a coding capacity of 315 amino acids (Mr 34 299), Mapping of the mRNA 5′ end showed that transcription starts 138 nucleotides upstream from the initiation codon. Upstream from the manX-coding region a possible promoter and ribosome binding site were identified. The manX gene is closely linked to the manY(pel) gene, the genes being transcribed in the same direction probably as one transcriptional unit. No apparent sequence homology was found between manX, encoding the mannose-specific enzyme IIIMan, and crr, encoding the glucose-specific enzyme IIIGlc, suggesting a divergent origin of these functionally related proteins.
The 5' ends of dihydrofolate reductase (DHFR)-specific transcripts have been mapped in the 5'-flanking region of the amplified DHFR gene of the human methotrexate-resistant cell line 6A3 by primer extension and S1 protection experiments. The main 5' end, at position -71 relative to the first nucleotide of the DHFR reading frame, corresponds to the recently identified main transcription initiation site for the DHFR gene and pertains to transcripts representing approximately 99% of the DHFR-specific polysomal polyadenylic acid-containing RNA, and including the previously described DHFR mRNAs wit
The classical scheme for the synthesis of double-stranded cDNA as it was reported in 1976 is described. Reverse transcription of mRNA with oligo(dT) as the primer generates first strands with a small loop at the 3' end of the cDNA (the end that corresponds to the 5' end of the mRNA). Subsequent removal of the mRNA by alkaline hydrolysis leaves single-stranded cDNA molecules again with a small 3' loop. This loop can be used by either reverse transcriptase or Klenow fragment of DNA polymerase I as a primer for second-strand synthesis. The resulting products are double-stranded cDNA molecules tha
Everything we examined (4)
This check searched the claim as stated. It did not run a separate search for evidence against it.