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the claim
The distance between the ribosome binding site and start codon is critical for prokaryotic translation efficiency
the verdict
SUPPORTED
the evidence backs this
confidence 84/100

The distance between the ribosome binding site (Shine-Dalgarno sequence) and the start codon is critical for prokaryotic translation efficiency, as demonstrated by studies showing that improper spacing destabilizes tRNA binding and reduces translocation rates.

Evidence for · 2
Short spacing between the Shine–Dalgarno sequence and P codon destabilizes codon–anticodon pairing in the P site to promote +1 programmed frameshifting
2010 · cited by 22
Paper 1 demonstrates that altering the spacing between the Shine-Dalgarno sequence and the P codon heavily impacts P-site tRNA binding and complex formation.
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More for · 1
Extending the spacing between the Shine-Dalgarno sequence and P-site codon reduces the rate of mRNA translocation
2020 · cited by 0
Paper 3 shows that the length of the spacer between the Shine-Dalgarno sequence and the codon strongly affects ribosome translocation and mRNA-ribosome interactions.
The paper trail · every fact has a biography
first checked01 Aug 2026
judged → SUPPORTED · 8401 Aug 2026
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