Specific DNA sequence characteristics influence the transcription efficiency of T7 RNA polymerase
the verdict
SUPPORTED
the evidence backs this
confidence 86/100
Evidence from multiple studies confirms that specific DNA sequence characteristics, such as promoter elements and regional modifications, directly influence the transcription efficiency and activity of T7 RNA polymerase.
Evidence for · 2
Entropy-driven assisted T7 RNA polymerase amplification-activated CRISPR/Cas13a activity for SARS-CoV-2 detection in human pharyngeal swabs and environment by an electrochemiluminescence biosensor.
2023 · cited by 70
This study demonstrates that the recognition of specific promoter sequences on DNA templates by T7 RNA polymerase is essential for initiating transcription.
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More for · 1
A novel analytical principle using AP site-mediated T7 RNA polymerase transcription regulation for sensing uracil-DNA glycosylase activity.
2020 · cited by 5
This study demonstrates that specific modifications within the T7 promoter sequence, such as abasic sites, can either inhibit or enhance transcription efficiency depending on their position.