IC50 values vary in reproducibility and trustworthiness across different experimental assays.
the verdict
SUPPORTED
the evidence backs this
confidence 92/100
Evidence from multiple studies indicates that IC50 values and assay reproducibility fluctuate depending on the specific experimental assay, readout method, and substrate used.
Evidence for · 3
Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays
2015 · cited by 487
The study demonstrates that different cell enumeration assays (such as MTT, NRU, RES, and SRB) exhibit varying degrees of variability and reproducibility when determining IC50 values.
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More for · 2
Cell-Based Luciferase Assay for Testing SARS-CoV-2 3CL Protease Inhibitors.
2026 · cited by 0
The research shows that IC50 values obtained for inhibitors differ significantly between cell-based and cell-free assays due to cellular barriers.
Label-Free High-Throughput Screening of CYP3A4 Inhibitors Using Acoustic Ejection Mass Spectrometry.
2026 · cited by 0
Findings confirm that both the assay method and the chosen substrate influence which inhibitor hits are detected and their measured potency.