Human DNA can be successfully sequenced from wastewater treatment plants
Multiple studies demonstrate that human and microbial DNA can be successfully extracted, sequenced, and analyzed from wastewater treatment plant samples for surveillance purposes.
The claim is specific, empirical, and testable. Several retrieved papers (such as papers 0, 6, 10, and 11) explicitly describe the extraction, sequencing, or detection of human and microbial DNA directly from municipal wastewater influent samples. Therefore, the evidence strongly supports the claim.
Wataru Hayashi, Hayato Tanaka, Yui Taniguchi, Masaki Iimura, Eiji Soga, R. Kubo, Nao Matsuo, K. Kawamura, Y. Arakawa, Y. Nagano, N. Nagano. Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan. 2019. https://doi.org/10.1128/AEM.01661-19
Paper 0 performs whole-genome sequencing on bacterial DNA isolated from municipal wastewater influents.
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Liang Peng, Fengxia Yang, Jingliang Shi, Yixin Liu, Liuzhu Pan, Daqing Mao, Yi Luo. Insights into the panorama of multiple DNA viruses in municipal wastewater and recycled sludge in Tianjin, China.. 2024. https://doi.org/10.1016/j.envpol.2024.124215
Paper 6 uses metagenomic sequencing to identify and track DNA viruses, including human viral families, in municipal wastewater.
Kevin K Chau, T Goodall, M Bowes, K Easterbrook, H Brett, J Hughes, D W Crook, D S Read, A S Walker, N Stoesser. High-resolution characterization of short-term temporal variability in the taxonomic and resistome composition of wastewater influent.. 2023. https://doi.org/10.1099/mgen.0.000983
Paper 10 extracts metagenomic DNA from wastewater influent for taxonomic profiling and resistome analysis.
Zhugen Yang, Marc Anglès d'Auriac, Sean Goggins, Barbara Kasprzyk-Hordern, Kevin V Thomas, Christopher G Frost, Pedro Estrela. A novel DNA biosensor using a ferrocenyl intercalator applied to the potential detection of human population biomarkers in wastewater.. 2015. https://doi.org/10.1021/acs.est.5b00637
Paper 11 extracts and detects human-specific mitochondrial DNA from wastewater to evaluate population biomarkers.
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