Freezing microorganisms such as probiotics kills them
the verdict
SUPPORTED
the evidence backs this
confidence 75/100
Freezing microorganisms such as probiotics can induce significant cellular damage and mortality through intracellular ice formation and osmotic stress, though survival can be improved using cryoprotectants and specialized drying protocols.
Evidence for · 8
Влияние криопротекторов на выживаемость заквасочных микроорганизмов при сублимационной сушке
2025 · cited by 0
This study discusses the survival of starter cultures during freeze-drying, which depends on their resistance to freezing and the use of cryoprotectants to mitigate damage.
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More for · 7
The effect of freeze-drying and storage on lactobacilli strains isolated from the gastrointestinal tract of wild boars using different cryoprotectants
2026 · cited by 0
This paper evaluates the viability of lactobacilli strains after freeze-drying, noting significant cell loss unless protected by specific media.
Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells.
2014 · cited by 0
This study evaluates probiotic survival after freeze-drying, showing very low survival rates for free cells without protective encapsulation.
Efficacy of the incorporation between self-encapsulation and cryoprotectants on improving the freeze-dried survival of probiotic bacteria.
2022 · cited by 0
This research aims to improve the viability of probiotic bacteria during freeze-drying by combining techniques to overcome freezing-induced stress and death.
Lactobacillus, Bifidobacterium and Lactococcus response to environmental stress: Mechanisms and application of cross-protection to improve resistance against freeze-drying.
2022 · cited by 0
This review highlights that lower survival rates of lactic acid bacteria are linked to freeze-drying and environmental stress, requiring adaptive mechanisms.
Inactivation mechanisms of lactic acid starter cultures preserved by drying processes.
2008 · cited by 0
This paper reviews inactivation mechanisms during drying processes, noting that freezing temperatures cause cell injuries due to solution effects and intracellular ice formation.
Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying.
2020 · cited by 0
This study examines Propionibacterium freudenreichii survival during drying processes like freeze-drying, where minimizing bacterial death is a major technological challenge.
Benefits and drawbacks of osmotic adjustment in Propionibacterium freudenreichii.
2019 · cited by 0
This report discusses the stresses encountered during freeze-drying of beneficial bacteria, which can reduce viability unless mitigated by proper osmoadaptation and protective agents.