Freezing microorganisms such as probiotics kills them
Freezing microorganisms such as probiotics can induce significant cellular damage and mortality through intracellular ice formation and osmotic stress, though survival can be improved using cryoprotectants and specialized drying protocols.
The claim states that freezing microorganisms such as probiotics kills them. The retrieved literature consistently demonstrates that freezing and freeze-drying processes induce cell injuries and death in probiotics due to ice crystal formation and osmotic stress. However, many studies also explore how to mitigate this mortality using cryoprotectants, encapsulation, or stress adaptation. Because unmitigated freezing reliably causes substantial cell death and requires special protective measures to preserve viability, the evidence clearly supports the claim.
И.А. Бурков, В.А. Митрова, А.Е. Рябова. Влияние криопротекторов на выживаемость заквасочных микроорганизмов при сублимационной сушке. 2025. https://doi.org/10.52653/ppi.2025.5.5.027
This study discusses the survival of starter cultures during freeze-drying, which depends on their resistance to freezing and the use of cryoprotectants to mitigate damage.
See more details
Katerina Kavanova, Vladimir Babak, Magdalena Crhanova. The effect of freeze-drying and storage on lactobacilli strains isolated from the gastrointestinal tract of wild boars using different cryoprotectants. 2026. https://doi.org/10.21203/rs.3.rs-9480160/v1
This paper evaluates the viability of lactobacilli strains after freeze-drying, noting significant cell loss unless protected by specific media.
Huiyi Song, Weiting Yu, Xiudong Liu, Xiaojun Ma. Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells.. 2014. https://doi.org/10.1016/j.carbpol.2014.02.084
This study evaluates probiotic survival after freeze-drying, showing very low survival rates for free cells without protective encapsulation.
Thi-Tho Nguyen, Phu-Tho Nguyen, Thi-To-Uyen Nguyen, Thi-Bich-Nhu Nguyen, Nhi-Binh Bui, Huu-Thanh Nguyen. Efficacy of the incorporation between self-encapsulation and cryoprotectants on improving the freeze-dried survival of probiotic bacteria.. 2022. https://doi.org/10.1111/jam.15473
This research aims to improve the viability of probiotic bacteria during freeze-drying by combining techniques to overcome freezing-induced stress and death.
Xinwei Gao, Jie Kong, Hongkang Zhu, Bingyong Mao, Shumao Cui, Jianxin Zhao. Lactobacillus, Bifidobacterium and Lactococcus response to environmental stress: Mechanisms and application of cross-protection to improve resistance against freeze-drying.. 2022. https://doi.org/10.1111/jam.15251
This review highlights that lower survival rates of lactic acid bacteria are linked to freeze-drying and environmental stress, requiring adaptive mechanisms.
C Santivarangkna, U Kulozik, P Foerst. Inactivation mechanisms of lactic acid starter cultures preserved by drying processes.. 2008. https://doi.org/10.1111/j.1365-2672.2008.03744.x
This paper reviews inactivation mechanisms during drying processes, noting that freezing temperatures cause cell injuries due to solution effects and intracellular ice formation.
Floriane Gaucher, Houem Rabah, Koffigan Kponouglo, Sylvie Bonnassie, Sandrine Pottier, Anne Dolivet, Pierre Marchand, Romain Jeantet, Philippe Blanc, Gwénaël Jan. Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying.. 2020. https://doi.org/10.1007/s00253-020-10425-1
This study examines Propionibacterium freudenreichii survival during drying processes like freeze-drying, where minimizing bacterial death is a major technological challenge.
Floriane Gaucher, Sylvie Bonnassie, Houem Rabah, Pauline Leverrier, Sandrine Pottier, Julien Jardin, Valérie Briard-Bion, Pierre Marchand, Romain Jeantet, Philippe Blanc, Gwénaël Jan. Benefits and drawbacks of osmotic adjustment in Propionibacterium freudenreichii.. 2019. https://doi.org/10.1016/j.jprot.2019.103400
This report discusses the stresses encountered during freeze-drying of beneficial bacteria, which can reduce viability unless mitigated by proper osmoadaptation and protective agents.
The paper trail · every fact has a biography
Challenge the receipt
Citation formatting by citeproc-js (Frank Bennett) and the Citation Style Language project. Source and licenses.
Terms · Privacy · How verdicts work · Dispute this receipt