Ethidium bromide contamination can be effectively degraded using a sodium nitrite and hypophosphorous acid solution.
the verdict
SUPPORTED
the evidence backs this
refutedsupported
the weight of evidence
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Peer-reviewed literature demonstrates that ethidium bromide solutions can be completely degraded by reaction with sodium nitrite and hypophosphorous acid, producing non-mutagenic reaction mixtures.
Ethidium bromide in water, TBE buffer, Mops buffer, and cesium chloride solution may be completely degraded by reaction with sodium nitrite and hypophosphorous acid. Only non-mutagenic reaction mixtures were produced. Destruction was greater than 99.8% in all cases; the limit of detection was 0.5 micrograms ethidium bromide per milliliter of solution. Ethidium bromide also may be removed completely from the above solutions by using Amberlite XAD-16 resin. The limit of detection was 0.05 micrograms ethidium bromide per milliliter of solution (0.27 micrograms/ml when cesium chloride solution was used).
Ethidium bromide: destruction and decontamination of solutions.
Ethidium bromide in water, TBE buffer, Mops buffer, and cesium chloride solution may be completely degraded by reaction with sodium nitrite and hypophosphorous acid. Only non-mutagenic reaction mixtures were produced. Destruction was greater than 99.8% in all cases; the limit of detection was 0.5 micrograms ethidium bromide per milliliter of solution. Ethidium bromide also may be removed completely from the above solutions by using Amberlite XAD-16 resin. The limit of detection was 0.05 micrograms ethidium bromide per milliliter of solution (0.27 micrograms/ml when cesium chloride solution was used).
Published in Analytical biochemistry (1987)
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