The successful application of honey in wound care management has been achieved due to honey's potent antibacterial effects, characterised by its multifactorial action. Impressive clinical efficacy has ignited its further use in diverse clinical disciplines, including stomatology. Indeed, there is increasing usage of honey in dental medicine as a preventive or therapeutic remedy for some periodontal diseases mainly associated with bacteria, such as dental caries, gingivitis and mucositides. Dental caries is undoubtedly a major oral health problem worldwide, with an increasing tendency of incidence. The purpose of this perspective review is to describe the recent progress in the laboratory and clinical use of honey in the prevention of dental caries, with emphasis on the antibacterial and antibiofilm effects of honey. The role of honey in the cariogenic process is also discussed. In addition, the quality of honey and the urgent in vitro evaluation of its antibacterial/antibiofilm properties before clinical use are highlighted. Findings based on data extracted from laboratory studies demonstrate the pronounced antibacterial effect of different honeys against a number of periodontal pathogens, including <i>Streptococcus mutans</i>. Although the promising antibiofilm effects of honey have been reported mainly against <i>S. mutans</i>, these results are limited to very few studies. From a clinical point of view, honey significantly reduces dental plaque; however, it is not superior to the conventional agent. Despite the positive in vitro results, the clinical effectiveness of honey in the prevention of dental caries remains inconclusive since further robust clinical studies are needed. Indeed, there is increasing usage of honey in dental medicine as a preventive or therapeutic remedy for some periodontal diseases mainly associated with bacteria, such as dental caries, gingivitis and mucositides. Dental caries is undoubtedly a major oral health problem worldwide, with an increasing tendency of incidence. The purpose of this perspective review is to describe the recent progress in the laboratory and clinical use of honey in the prevention of dental caries, with emphasis on the antibacterial and antibiofilm effects of honey. The role of honey in the cariogenic process is also discussed. Despite the positive in vitro results, the clinical effectiveness of honey in the prevention of dental caries remains inconclusive since further robust clinical studies are needed. Keywords: honey, antibacterial, quality, dental medicine, S. mutans status released display-pdf yes is-olf no is-manuscript no is-preprint no is-journal-matter no is-scanned no is-retracted no Received 2022 Aug 9; Accepted 2022 Sep 1; Collection date 2022 Sep. 1. Introduction Dental caries is considered to be a global health priority in the treatment of oral diseases. According to the Global Burden of Disease 2015 study, 3.5 billion people worldwide had dental conditions, predominantly untreated dental caries [ 1 ]. Overall, the global burden of untreated dental caries for primary and permanent dentition has remained relatively unchanged over the past 30 years [ 2 ]. Dental caries is the localised destruction of susceptible dental hard tissues (enamel and dentine) by weak organic acidic by-products from the bacterial fermentation of dietary carbohydrates (sucrose) [ 3 ]. A very recent epidemiological study showed that the global number of cases of caries of permanent teeth increased by 46.1% (95% uncertainty interval, 42.0% to 50.3%) from 1990 to 2019 [ 9 ]. The fast increase in the dental caries burden suggests the need to re-evaluate the current insurance scheme and strengthen efforts to prevent caries. It has recently been suggested that dental caries prevention and management is about controlling risk factors to maintain a balanced intraoral biofilm ecology that guards against a continuing low pH [ 10 ]. Based on current knowledge, the authors classified dental caries as a non-communicable disease. 3.2. Antibiofilm Effect of Honey against Oral Pathogens Dental biofilms play a vital role in caries initiation and development. Therefore, the prevention and/or eradication of dental plaque biofilm is an important approach in the management of dental caries. There are several strategies for inhibiting the formation of cariogenic bacterial biofilms as well as for disrupting already-formed biofilm [ 59 ]. From practical and economic points of view, the prevention of plaque biofilm formation is a key step in maintaining good oral health. Several types of polyphenols have antimicrobial properties and can inhibit bacterial growth, adherence and biofilm formation (reviewed in [ 76 ]). Data from clinical studies show In contrast, rinsing with 10% sucrose solution for 1 min caused a decrease in pH value to below 5.5. In addition, bacterial counts were significantly reduced only in the honey group compared to the other treatment groups. The dental plaque score in 56 subjects who used a 5% honey solution as a mouthwash for a period of 6 days was determined by Alibasyah et al. (2018) [ 86 ]. A significant difference between dental plaque score before and after 6 days of using honey solution was found. Honey is often used as a mouthwash in the clinical testing of the treatment of periodontal diseases [ 89 ]. Therefore, the quality of honey and its antibacterial and antibiofilm activity need to be determined before pre-clinical and clinical trials are conducted. The antibacterial, and particularly antibiofilm, effect of honey is an important factor in determining honey’s clinical efficacy in the prevention of dental caries through the eradication of viable bacteria within dental plaque biofilm. We argue that the information presented in this perspective review will stimulate further clinical research on honey in periodontal diseases where its antibacterial/antibiofilm as well as anti-inflammatory and wound healing activities will be employed.