Achieving sterility in nucleic acid work requires specific enzymatic and chemical decontamination
the verdict
SUPPORTED
the evidence backs this
confidence 84/100
Achieving and maintaining sterility or freedom from contamination in nucleic acid work frequently requires specialized enzymatic and chemical decontamination protocols, such as uracil-DNA-glycosylase or restriction enzyme treatments.
Evidence for · 3
A CRISPR/Cas9 eraser strategy for contamination‐free PCR end‐point detection
2021 · cited by 30
Demonstrates the use of a CRISPR/Cas9 strategy to eliminate contamination in PCR systems.
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More for · 2
Elimination of Carryover Contamination in Real-Time Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid Detection of the SARS-CoV-2 Virus in Point-of-Care Testing
2022 · cited by 14
Shows the use of Cod-uracil-DNA-glycosylase to eliminate carryover contamination in nucleic acid amplification assays.
Simple enzymatic means to neutralize DNA contamination in nucleic acid amplification.
2005 · cited by 0
Describes enzymatic methods such as restriction enzymes to neutralize DNA contamination in nucleic acid amplification.